| PCR 기법을 이용한 복제가능 B형 간염바이러스 이량체 게놈의 검정 ( Identification of eplication-Competent Dimeric Human Hepatitis B Viral Using PCR Screening Technique ) |
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| Identification of eplication-Competent Dimeric Human Hepatitis B Viral Using PCR Screening Technique |
| Seok Hyun Nam, Ph.D.1, Sung Won Cho, M.D.2 |
| Department of Biological Science, College of Natural Sciences, Ajou University1,
Department of Gastroenterology, Ajou University School of Medicine2, Suwon, Korea |
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| ABSTRACT |
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Background/Aims
Transfection of the hepatitis B virus (HBV) genome requires the cloning of tandem HBV sequences inserted into a plasmid vector, which is usually screened for by the restriction enzyme digestion of plasmid minipreparation from at least a dozen of bacterial colonies. The aim of this study was to develop a simple alternative screening method for bacterial colonies harbouring tandem HBV sequences by a PCR. Methods: A set of polymerase chain reaction (PCR) primer was designed to detect the bacterial colonies harbouring "head to tail" dimeric HBV DNA. PCR which amplifies the head to tail junction site of two tandem HBV molecules was performed. Results: PCR products with appropriate size (1.2kb) were obtained. The accurate detection by PCR screening technique was confirmed by enzyme digestion. Conclusions: These results suggest that PCR screening technique is a simple and rapid method for the identification of bacterial colonies containing tandem HBV sequences. (Korean J Hepatol 1999;5:184－189) |
| KeyWords:
Tandem HBV sequences, PCR, Rapid screening |
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