Resection plane-dependent error in computed tomography volumetry of the right hepatic lobe in living liver donors

Heon-Ju Kwon; Kyoung Won Kim; Bohyun Kim; So Yeon Kim; Chul Seung Lee; Jeongjin Lee; Gi Won Song; Sung Gyu Lee

doi:10.3350/cmh.2017.0023

Clin Mol Hepatol > Volume 24(1); 2018 > Article

Kwon, Kim, Kim, Kim, Lee, Lee, Song, and Lee: Resection plane-dependent error in computed tomography volumetry of the right hepatic lobe in living liver donors

Original Article

Clin Mol Hepatol. 2018; 24(1): 54-60.

Published online: August 1, 2017

DOI: https://doi.org/10.3350/cmh.2017.0023

Resection plane-dependent error in computed tomography volumetry of the right hepatic lobe in living liver donors

Heon-Ju Kwon¹, Kyoung Won Kim²

, Bohyun Kim³, So Yeon Kim², Chul Seung Lee², Jeongjin Lee⁴, Gi Won Song⁵, Sung Gyu Lee⁵

Corresponding author : Kyoung Won Kim Department of Radiology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea
Tel: +82-2-3010-4385, Fax: +82-2-476-4719 E-mail: kimkw@amc.seoul.kr

Received April 8, 2017 Revised June 24, 2017 Accepted June 27, 2017

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

See the commentary-article "Clinical implication of hepatic volumetry for living donor liver transplantation" on page 51.

ABSTRACT

Background/Aims

Computed tomography (CT) hepatic volumetry is currently accepted as the most reliable method for preoperative estimation of graft weight in living donor liver transplantation (LDLT). However, several factors can cause inaccuracies in CT volumetry compared to real graft weight. The purpose of this study was to determine the frequency and degree of resection plane-dependent error in CT volumetry of the right hepatic lobe in LDLT.

Methods

Forty-six living liver donors underwent CT before donor surgery and on postoperative day 7. Prospective CT volumetry (V_P) was measured via the assumptive hepatectomy plane. Retrospective liver volume (V_R) was measured using the actual plane by comparing preoperative and postoperative CT. Compared with intraoperatively measured weight (W), errors in percentage (%) V_P and V_R were evaluated. Plane-dependent error in V_P was defined as the absolute difference between V_P and V_R. % plane-dependent error was defined as follows: |V_P–V_R|/W∙100.

Results

Mean V_P, V_R, and W were 761.9 mL, 755.0 mL, and 696.9 g. Mean and % errors in V_P were 73.3 mL and 10.7%. Mean error and % error in V_R were 64.4 mL and 9.3%. Mean plane-dependent error in V_P was 32.4 mL. Mean % plane-dependent error was 4.7%. Plane-dependent error in V_P exceeded 10% of W in approximately 10% of the subjects in our study.

Conclusions

There was approximately 5% plane-dependent error in liver V_P on CT volumetry. Plane-dependent error in V_P exceeded 10% of W in approximately 10% of LDLT donors in our study. This error should be considered, especially when CT volumetry is performed by a less experienced operator who is not well acquainted with the donor hepatectomy plane.

Keywords: Liver transplantation; Living Donors; Volumetric computed tomography; Organ volume; Liver

INTRODUCTION

Living donor liver transplantation (LDLT) is a widely used option for the treatment of patients with end-stage liver diseases. To meet the large metabolic demand of adult recipients, the right hepatic lobe is preferred rather than the left hepatic lobe as a graft in adult-to-adult LDLT [1,2]. In order to use a right hepatic lobe as a graft for LDLT, an exact pre-operative assumption of the graft weight is the essential step, because a ‘small-for-size’ graft in which the graft-to-recipient weight ratio is less than 0.8-1% may result in graft malfunction or metabolic insufficiency for the recipient and because over-resection of the donor liver may threaten the donor’s safety [3-8].

Computed tomography (CT) hepatic volumetry is currently accepted as the most reliable method for preoperative assumption of the graft weight for living donor liver transplantation [9-20]. However, the estimated value from CT volumetry can be deviated from the real graft weight due to several factors, including (a) a difference between the preoperative assumptive and the actual hepatectomy planes, (b) the blood volume contained in the large hepatic vessels [2,9-11], and (c) the variable physiological density of the liver graft [12,13]. It may therefore be worthwhile to determine how much each factor contributes to the error of CT volumetry. In our institution, we routinely obtain CT scans on postoperative day 7 in donors, as well as recipients, for the screening of postoperative complications. Therefore, we assumed that the actual resection planes for the procurement of the right hepatic lobe can be determined using postoperative CT scans in the donor and the recipient as a reference, and thereby the error of CT volumetry due to the difference between the preoperative assumptive and the actual hepatectomy planes can be assessed.

Thus, the purpose of this study was to determine the resection plane-dependent error of CT volumetry for right hepatic lobe in live liver donors.

MATERIALS AND METHODS

Study population

This study was approved by our institutional review board (2014-1012). They permitted waiving informed consent from study subjects for this retrospective study.

The study group consisted of 46 live liver donors (mean age, 27.0 years ± 8.2; range, 16-46 years; 30 men [25.5 years ± 7.3; 16-41 years], 16 women [27.0 years ± 8.2; range, 16-41 years]) who donated their right hepatic lobe for LDLT from February to March 2014 in our institution. The donors underwent CT scans of the liver as a preoperative work-up for LDLT within 2 months prior to liver procurement. They also underwent CT scans for the screening for surgical complications on postoperative day 7. The recipients (mean age, 50.0 years ± 8.0; range, 31-67 years) consisted of 35 men (50.0 years ± 8.1; range, 31-67 years) and 11 women (50.2 years ± 8.0; range, 31-67 years). Mean body weight was 66.6 kg ± 10.5 (range, 43.0-89.0 kg). They also underwent CT scans for the screening of surgical complications at least once within postoperative day 7.

CT protocols

Preoperative CT scans were obtained with a 64-channel MDCT scanner (Somatom Definition; Siemens Medical Solutions, Erlangen, Germany). The donors fasted for more than 8 hours before CT. CT was performed with a breath hold at the end of expiration. After obtaining unenhanced CT scans, 150 mL of iopromide (Ultravist 370; Bayer Schering Pharma, Berlin, Germany) was administered at a flow rate of 3 mL/sec using a mechanical injector. Biphasic CT scanning was then performed during the hepatic arterial phase (HAP) and the venous phase (VP). By means of a bolus-tracking method (Smart Prep; GE Healthcare or CARE-Bolus; Siemens Medical Solutions), HAP scanning was initiated at 10 seconds after enhancement of the descending aorta reached 100 HU. V_P scanning, targeted for good opacification of both the portal and hepatic veins, was initiated 40 seconds after HAP scanning. The scanning parameters for preoperative CT scans were as follows: detector collimation of 0.6 mm × 64; table pitch of 1; gantry rotation time of 0.5 sec; 200 effective mAs; and 100 kVp. Images were reconstructed with a 5-mm slice thickness at a 5-mm interval, and were then downloaded to the picture archiving and communication system. In addition, the images were reconstructed at 1-mm section thicknesses at 0.70-mm intervals, and were downloaded to a workstation (Advantage Windows, version 4.6; GE Medical Systems, Chalfont, UK) for 3-dimensional reconstruction of the hepatic vessels. Maximum intensity projection and volume-rendering techniques were used as the standard algorithms.

Postoperative CT scans for recipients were obtained in a similar manner to that of preoperative donor CT scans. Postoperative post-contrast CT scans for donors were obtained using a low-dose protocol (50 effective mAs and 100 kVp).

CT volumetry

CT volumetry of the right hepatic lobe was performed twice per donor prospectively (before liver procurement) and retrospectively (3 months after liver procurement). We used the computer-aided in-house liver volumetry software for our study, using VP images of preoperative donor CT scans. The overall procedure for semiautomated liver volumetry is as follows. Firstly, modified curvature diffusion filtering was applied to smooth the original CT image while preserving the edge. Secondly, the initial liver outline was detected through the sequential applications of seeded region growing onto level-set speed images, which was generated as a map inversely proportional to the gradient magnitude [21]. Thirdly, the level-set method performed liver segmentation based on the initially detected liver contour [21]. Malladi’s level-set method was adopted for level-set propagation [22]. Then, a rolling ball algorithm and the removal of false positives were performed to enhance the liver boundary more accurately. Finally, the radiologist defined a resection plane dividing the liver into right and left hepatic lobes on individually selected slices by inputting 2 end points of the line. When resection lines on at least 2 slices were available, all resection lines on intervening slices in between could be computed utilizing the interpolation technique.

For prospective CT volumetry (V_P), resection plane for the procurement of the right hepatic lobe was determined by 2 radiologists in consensus after the training session (CS Lee, BH Kim>500 cases, each), based on the right border of the middle hepatic vein superiorly and the Cantlie line (imaginary line between the gall-bladder and the inferior vena cava) inferiorly, also using the maximum intensity projections of VP images for reference. Then, the volume of the right hepatic lobe was automatically calculated using a summation-of-area method [14].

Retrospective CT volumetry (V_R) was performed 3 months after the liver procurement in order to avoid recall bias. The reviewers were blinded to the V_P and the intraoperative weight (W) of the right-lobe graft. For reference, postoperative CTs (≤ postoperative day 7) of donor and recipient were carefully compared to determine the actual resection planes for the procurement of the right hepatic lobe. Also, by applying the surgical resection plane inferred from the postoperative CTs to the CT volumetry, the V_R was obtained. The other procedures of volumetry were the same as those of V_P.

Intraoperative weight measurement

The right hepatic lobes were procured from each donor by several liver transplantation surgeons. After the procurement of the graft, they shook the excised graft to spill out the remained blood in the large vessels and then measured the weight of the graft on a back table using an electronic laboratory scale. After the weight measurement, they flushed the procured graft via the portal vein with histidine-tryptophan-ketoglutarate solution.

Statistical analysis

All statistical analyses were performed using commercially available software (Medcalc, Version 16.1; Frank Schoonjans, Mariakeke, Belgium). The significance was defined as P<0.05.

Pearson correlation tests were performed to determine the correlation coefficients between V_P or V_R and W.

The following indexes were calculated to measure the deviations of V_P and V_R from W. The errors of V_P and V_R were defined as the absolute amount of deviation of V_P and V_R from W. The percentage (%) errors were defined as the errors of V_P and V_R divided by W and multiplied by 100. The plane-dependent error of V_P was defined as the absolute amount of difference between V_P and V_R. The % plane-dependent error of V_P was defined as the plane-dependent error of V_P divided by W and multiplied by 100. The following are the Equations:

(1)

Error of VP=Vp-W

(2)

Error of VR=VR-W

(3)

% Error of VP=VP-W/W·100

(4)

% Error of VR=VR-W/W·100

(5)

Plane-dependent Error of VP=VP-VR

(6)

% Plane-dependent Error of VP=VP-VR/W·100

The difference in graft volume/recipient body-weight ratios using V_P and V_R were evaluated by paired t-test. The difference in the frequency of small-for-size graft (graft volume/recipient body-weight ratio <0.8) was evaluated.

RESULTS

In our study, the V_P and V_R ranged from 469.3 to 1,258.6 mL and from 462.2 to 1,237.6 mL, respectively. The W varied between 400.0 g and 1,050.0 g. The mean ± standard deviation, median, and 25th and 75th percentiles are summarized with their 95% confidence intervals in Table 1, and are depicted in box and whisker plots (Fig. 1).

V_P strongly correlated with W with r=0.8898 (95% confidence interval, 0.8082 to 0.9378; P<0.001). The V_P showed a deviation of -60.9 to 208.6 mL from W. The V_P led to an overestimation compared with W in 39 (84.8%) live liver donors and an underestimation in the other 7 (15.2%). The error of V_P ranged from 0.7 to 208.6 mL, and the % error of V_P was between 0.1% and 32.3%. A >10% error occurred in 23 of 46 donors (50%).

V_R also strongly correlated with W with r=0.9192 (95% confidence interval, 0.8578 to 0.9547; P<0.0001). The V_R showed a deviation of -43.1 to 187.6 mL from W. The V_R was overestimated compared with W in 38 (82.6%) and underestimated in 8 (17.4%) of 46 live liver donors. The error of V_R ranged from 0.1 to 187.6 mL, and the mean % error of V_R was between 0.01% and 25.1%. A >10% error occurred in 19 (41.3%) subjects.

Compared with V_R, V_P deviated from -102.1 to 112.6 mL (Fig. 2). V_P was larger than V_R in 28 (60.9%), and smaller than V_R in the other 18 (39.1%). The plane-dependent error of V_P was from 1.6 to 112.6 mL. The % plane-dependent error of V_P was between 0.2% and 18.2%. The plane-dependent error exceeded 10% compared with W in approximately 10% (n=5) of the subjects in the prospective setting.

The mean ± standard deviation, median, and 25th and 75th percentiles of error of V_P and V_R, % error of V_P and V_R, and the plane-dependent error and % plane-dependent error of V_P, are summarized with their 95% confidence intervals in Table 2.

Graft volume/recipient body-weight ratios according to V_P was 1.16 ± 0.24% and that according to V_R was 1.16 ± 0.26%, respectively (P=0.382). There was no small-for-size graft according to V_P and V_R.

DISCUSSION

Among several factors that may cause the error in CT volumetry in potential live liver donors for LDLT, we focused on the difference between the preoperative assumptive and the actual hepatectomy planes for right lobe procurement. This kind of error could be minimized if CT volumetry is performed by an expert operator (either a radiologist or surgeon), who is fully aware of the donor hepatectomy plane and the possible tailoring transection of segment V vein for optimal sharing of the middle hepatic vein [15]. However, such problems may occur to a larger extent when the procedure is performed by a less experienced operator. There may be some discrepancy between the line drawn through the middle hepatic vein and the margin of the area of right and left hemiliver, which may curve, undulate, or even interdigitate [23]. However, the frequency and the degree of such resection plane-dependent errors have not been acknowledged in the prospective setting. In our study, V_P showed a 10.7% difference on average compared to W. When we calculated the absolute difference of V_P from V_R (by applying the actual resection plane), the difference corresponded to 4.7% of W. Therefore, we suggest that CT volumetry in the prospective setting may produce about a 5% error, attributed to surgical plane mis-registration. The plane-dependent error of V_P exceeded 10% of W in approximately 10% of the subjects in our study.

Even with application of the actual resection plane, V_R showed considerable difference (9.3% ± 6.7) from W in our study. In >80% of the subjects, V_R was overestimated compared with W. Such a tendency of overestimation is consistent with that observed in previous studies [9,12,16,24,25]. The blood volume circulating in the large hepatic vessels is considered to be the major cause of overestimation of CT volumetry [26]. It has been shown that the blood pool was >20% of the whole liver volume in one animal study [10]. Similarly, Hwang et al. [11] also suggested that blood-filled grafts contain approximately 29 mL of blood per 100g. Therefore, this kind of error may be compensated for by applying a conversion factor between the graft volume and weight [12,27] or by using computer-aided liver volumetry that can assess the bloodless liver volume [9]. Another possible cause of error in V_R may be the variable physiological density of the hepatic graft, but the effect of physical density on the accuracy of CT volumetry must be further evaluated.

There are several limitations to this study. First, we did not assess the reproducibility of CT volumetry between operators or at different time points in our study. Also, it was not verified whether the resection plane-dependent error of CT volumetry varied depending on the experience of the operator. Therefore, a further study should be followed to determine the inter- and intra-observer variation in the performance of this technique. Second, although we carefully compared preoperative and postoperative CT scans in donors and postoperative CT scans in recipients to determine the actual resection plane, over or insufficient correction might have been possible, as the margin of the area of the right and left hemiliver may undulate or even interdigitate within the liver. We utilized the middle hepatic vein and the Cantlie line to divide the right and left hemiliver, but currently there are several commercially available softwares that provide the division of the right and left hemiliver based on the portal territory. While it is anticipated that resection plane-dependent error may decrease with this algorithm for liver division, the performance of these softwares should be analysed by further well-designed study.

In conclusion, CT volumetry in the prospective setting may produce about a 5% error, attributed to surgical plane mis-registration. The plane-dependent error of V_P exceeded 10% of W in approximately 10% of the subjects in our study. This amount of error should be considered in the prospective setting especially when CT volumetry is performed by a less experienced operator unacquainted with the donor hepatectomy plane.

ACKNOWLEDGMENTS

This research was supported by the Basic Science Research Program of the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology (grant 2010-0021107).

FOOTNOTES

Authors’ contribution

Dr. Kwon and Dr. KW Kim have full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.

- Study concept and design: Kwon and KW Kim.
- Acquisition of data: CS Lee, BH Kim, SY Kim, Song, and SG Lee.
- Analysis and interpretation of data: Kwon and JG Lee.
- Drafting of the manuscript: Kwon and KW Kim.
- Critical revision of the manuscript for important intellectual content: KW Kim, SY Kim, and SG Lee.
- Statistical analysis: Kwon, BH Kim, Song, and JG Lee.

FOOTNOTES

Conflicts of Interest: The authors have no conflicts to disclose.

Figure 1.

Box and whisker plot of prospective and retrospective computed tomography volumetry and intraoperatively-measured weight of the right hepatic lobe. The central boxes represent the lower to upper quartile (25th to 75th percentile) values. The middle lines represent the medians. Vertical lines extend from the minimum to the maximum values, excluding "outliers", which are displayed as separate points. The values plotted with a square marker are outliers (smaller than the lower quartile minus 1.5 times the interquartile range or larger than the upper quartile plus 1.5 times the interquartile range [inner fences]). V_P, prospective CT volumetry; V_R, retrospective CT volumetry; W, intraoperative weight.

Figure 2.

Computed tomography (CT) volumetry of the right hepatic lobe. (A) Prospective CT volumetry (V_P) for the right hepatic lobe was performed using the assumptive hepatectomy plane (green line), defined by the right border of the middle hepatic vein (MHV) and the Cantlie line (an imaginary line between the gallbladder and the inferior vena cava). The maximum intensity projection of the venous phase image was utilized as the reference. (B) Postoperative CT during the venous phase in the donor shows the three hepatic vein tributaries left in segment IV. The actual hepatectomy plane can be determined by careful comparison of postoperative CT between the donor and the recipient (not shown). (C) Retrospective CT volumetry (V_R) was performed using the actual hepatectomy plane (orange line). The plane-dependent error of V_P is the volume of the difference between V_P and V_R. LPV, left portal vein; RAPV, right anterior portal vein.

Table 1.

Summary statistics for semiautomated CT volumetry and intraoperative weight of the right hepatic lobe in 46 living liver donors

Volume or weight	Mean ± SD	Median	25^th percentile	75^th percentile
V_P (mL)	761.9 ± 136.6 (721.4, 802.5)	767.1 (731.1, 801.3)	681.1 (606.6, 742.1)	833.7 (783.9, 899.1)
V_R (mL)	755.0 ± 139.1 (713.7, 796.3)	765.1 (723.7, 789.1)	667.5 (580.0, 752.5)	826.8 (780.5, 883.9)
W (g)	696.9 ± 116.4 (662.4, 731.5)	705.0 (650.0, 750.0)	630.0 (558.7, 680.0)	770.0 (738.6, 800.0)

Numbers in parentheses are 95% confidence intervals.

CT, computed tomography; V_P, prospective CT volumetry; V_R, retrospective CT volumetry; W, intraoperative weight; SD, standard deviation.

Table 2.

V_P and V_R deviation compared with W of the right hepatic lobe in 46 living liver donors

	Mean ± SD	Median	25^th percentile	75^th percentile
Error of V_P (mL)	73.3 ± 52.4 (57.7, 88.8)	69.2 (37.0, 89.2)	28.1 (18.9, 41.5)	107.8 (83.5, 127.8)
Error of V_R (mL)	64.4 ± 48.5 (50.0, 78.8)	53.9 (37.2, 86.8)	17.5 (9.1, 42.8)	105.3 (77.8, 122.4)
% Error of V_P	10.7 ± 7.6 (8.5, 13.0)	10.0 (5.1, 13.0)	3.7 (2.7, 6.0)	16.4 (12.6, 19.5)
% Error of V_R	9.3 ± 6.7 (7.3, 11.3)	9.0 (5.8, 12.6)	2.7 (1.2, 6.1)	15.6 (12.5, 17.6)
Plane-dependent error of V_P	32.4 ± 27.0 (24.3, 40.4)	24.0 (19.2, 29.3)	13.7 (7.5, 19.8)	42.4 (29.0, 72.1)
% Plane-dependent error of V_P	4.7 ± 4.0 (3.5, 5.9)	3.3 (2.7, 4.1)	2.0 (1.3, 2.8)	6.7 (3.9, 9.3)

Numbers in parentheses are 95% confidence intervals

CT, computed tomography; V_P, prospective CT volumetry; V_R, retrospective CT volumetry; W, intraoperative weight; SD, standard deviation.

Abbreviations

HAP

hepatic arterial phase

LDLT

living donor liver transplantation

standard deviation

venous phase

V_P

prospective CT volumetry

V_R

retrospective CT volumetry

intraoperative weight

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